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How big is pBluescript?
Source/Vendor: | Stratagene |
---|---|
Plasmid Type | Bacterial Expression |
Cloning Method | Restriction Enzyme |
Size: | 2958 |
5' Sequencing 1 Primer: | M13pUC-fwd |
You may then wonder, "What is pBluescript?"
pBluescript is a good example of a combination of plasmids, phages (phagemids), This means that a cell that harbors a recombinant Phagemid will, if infected with the f1 helper virus that supplies single-stranded DNA replication components to phages, produce and package single-stranded Phagemid DNA.
You might also wonder what makes plasmid vectors like pBluescript useful cloning vectors. The majority of vectors have the basic nucleotide sequences necessary for DNA Cloning. These include a replication origin, drug-resistance genes, and an area in which exogenous DNA fragments may be inserted (Figure 7-1).
PBSK is simply that.
Pravasi Bhaktiya Sahayata Kendra ( /PBSK ), which was formerly known under the Indian Workers Resource Centre, reported a spike in workers not receiving their WPS dues.
What is the pUC19 plasmid?
pUC19, a low-copy E. coli plasmid cloning virus, has multiple cloning sites, as shown below. The molecule has a 2686 base pair length and is small, double-stranded. The ampicillin resistance gene confers selection of the plasmid for E. coli.
What is pBluescript SK+?
What is f1 origin?
What is a polylinker region?
What are the most important features that a plasmid vector possesses?
What are the 6 types of vectors?
- Plasmid. Circular extrachromosomal DNA that autonomously replicates inside the bacterial cell.
- Phage. Linear DNA molecules derived from bacteriophage lambda.
- Cosmids.
- Bacterial Artificial Chromosomes.
- Yeast Artificial Chromosomes.
- Human Artificial Chromosome.
What are the three key features of plasmid?
- Replication. Replication of plasmid DNA is carried by the same enzymes that replicate the E.
- Selectable Markers (Antibiotic resistance)
- Multiple cloning sites (or polylinkers)
- Single-stranded DNA production.
- Bacteriophage promoters.
- Miniature Preparation of Bacterial Plasmid.
What is vector and its types?
What is plasmid and its types?
What is a shuttle plasmid?
What is the structure of a plasmid?
What is lambda phage vector?
What is DNA cloning used for?
What are essential features of vectors?
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