Asked by: Crucita Eisen
Asked in category: science, biological sciences, science, biological sciences
Last Updated: 22nd May 2024
What is the use of Lactophenol cotton blue stain?
Fungal Stains. Lactophenol blue is a stains that can be used for fungal elements after a tape preparation or scraping. This stain contains phenol which kills the organisms, lactic acids which preserve fungal structures, as well as cotton blue which stains fungal cell walls.
Similar to the above, how can you make cotton blue Lactophenol.
Method
- The Cotton Blue should be dissolved in the distilled water on the first day.
- Wear gloves on the second day to add the phenol crystals and lactic acid to a glass beaker.
- Add the glycerol.
- Filter the Cotton Blue and distilled water solution into the phenol/glycerol/lactic acid solution.
What is the importance of Lactophenol Blue in observing fungi and what are its benefits? Lactophenol Cotton Blue can be used to stain fungi. Fungus is an eukaryotic organism. They are divided into two main groups: yeast and molds. Chitin is the main component of its cell wall. Fungal cells can have both a microscopic and macroscopic structure.
What stain is used to kill fungi?
The standard histological stains used to identify fungi are Grocott (methanmine), silver (GMS) stain and Periodic acid-Schiff. GMS stain is less sensitive than the PAS stain but it has a signal-to-noise issue because it stain inflammatory cells (lysosomes), tissue reticulin and fungi.
What are the main components of Lactophenol blue and what effect do they have?
This preparation contains three components: phenol which kills all living organisms; lactic which preserves fungal structures and cotton blue which stain the fungal cells walls.
17 Related Question Answers Found
What is the procedure for preparing a KOH mount?
Procedure to make 100 ml of KOH 20% w/v solution:
- Weigh 20 g potassium hydroxide (KOH) pellets.
- Transfer the chemical to a screw-cap bottle.
- Add 50 ml distilled water, and mix until the chemical is completely dissolved, add remaining distilled water and make the volume 100 ml.
- Label the bottle and mark it corrosive.
What is a tease mount?
Wet mount and tease mount are the easy and most preferred techniques by many laboratories. In this method, a small portion of a fungal colony is picked with one needle and a second needle is used to tease the fungus apart to form a thin layer. Then a cover slip is placed over the fungus for examination.
What is Lpcb in microbiology?
LPCB is a stain used for making semi-permanent microscopic preparation of fungi. The LPCB stain has following three components : Phenol : Kills any live organism. Lactic acid : Preserves fungal structures.
What is slide culture technique?
It is a rapid method of preparing fungal colonies for examination and identification. Fungi are identified mostly by close examination of its morphology and the characteristics it possess. In slide cultures, we are growing the fungi directly on the slide on a thin film of agar.
How do you make a fungi slide?
Slide Culture Preparations
- Using a sterile blade cut out an agar block (7 x 7 mm) small enough to fit under a coverslip.
- Flip the block up onto the surface of the agar plate.
- Inoculate the four sides of the agar block with spores or mycelial fragments of the fungus to be grown.
- Place a flamed coverslip centrally upon the agar block.
How is fungi used in industry?
Yeasts and other fungi play a critical role in drug production, food processing, bio-control agents, enzyme biotechnology, as well as research and development. The use of yeast (e.g. Saccharomyces cerevisae) to make alcohol and carbon dioxide uses the fermentation process to break down sugars.
Are fungi gram positive?
An example of gram-negative bacteria is Escherichia coli, the cause of many urinary tract infections. Fungi (in the form of yeasts or molds) can also be initially recognized with the Gram stain, but viruses cannot be seen with a Gram stain.
Why is Lactophenol used as mounting medium for molds and some fungi species?
Lactophenol along with Cotton Blue reagent is used for staining as well as for wet mounting of fungi(1). The preparation has three components: Lactic acid preserves the fungal structure and clears the tissue while phenol acts as a disinfectant and cotton blue imparts blue colouration to the fungal spores and hyphae(2).
How do you stain fungi?
Method of Use: Place a drop of Lactophenol Cotton Blue Stain in the center of a clean slide. Remove a fragment of the fungus colony 2-3mm from the colony edge using an inoculating or teasing needle or MycoMount™ adhesive strips (Cat. no.
What is GMS Stain used for?
In pathology, the Grocott-Gomori's (or Gömöri) methenamine silver stain, abbreviated GMS, is a popular staining method in histology. The stain was originally named after György Gömöri, the Hungarian physician who developed the stain. It is used widely as a screen for fungal organisms.
What is Giemsa stain used for?
It differentially stains human and bacterial cells purple and pink respectively. It can be used for histopathological diagnosis of malaria and some other spirochete and protozoan blood parasites. Giemsa stain is a classic blood film stain for peripheral blood smears and bone marrow specimens.
Do fungi have flagella?
Another feature of fungi is the presence of chitin in their cell walls. Most members of the kingdom Fungi lack flagella; the structures are completely absent in all stages of their life cycle. The only exception are the chytrids, which produce flagellated gametes.
How do you test for skin fungus?
The skin lesion KOH exam is a simple test that helps doctors identify fungal infections on a person's skin, hair, and nails. When doctors perform a KOH exam, they take a skin scraping, which they then place in a potassium hydroxide (KOH) solution and analyze under a microscope.
Why is Safranin used for staining?
Safranin is a biological dye that is used as a counterstain in many cytological and histological experiments. The dye leaves a red colour on the nuclei in the cells, which makes it easy to identify. Safranin stain is used in the preparation of temporary mount of stomata.
Why mycobacteria are acid fast?
These Acid-fast organisms like Mycobacterium contain large amounts of lipid substances within their cell walls called mycolic acids. These acids resist staining by ordinary methods such as a Gram stain. It can also be used to stain a few other bacteria, such as Nocardia. Acid-fast bacilli are bright red after staining.
Can fungi be seen under a microscope?
Molds. While there are many types of Fungi, MicroscopeMaster will focus on mold under the microscope here. Fungi is a taxonomic Kingdom that is composed of well over 99,000 species including yeast, molds, smuts and rusts among others. Like yeast, molds are also eukaryotic and can be found in moist places.
Which of the following stains is commonly used to stain chitin in fungal cell walls?
Fungi use chitin as structural material in their cell walls so the stain shows the cell wall well. The lactophenol cotton blue stain is the most common stain for fungi. The phenol kills microorganisms and prevents fungal enzymes from breaking down the cell. The cotton blue dye turns the chitin blue.
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